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open reading frame orf sequence  (TaKaRa)


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    TaKaRa open reading frame orf sequence
    Open Reading Frame Orf Sequence, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reading+frame+orf+sequences/pmc12870431-49-19-30?v=TaKaRa
    Average 99 stars, based on 2900 article reviews
    open reading frame orf sequence - by Bioz Stars, 2026-07
    99/100 stars

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    Dual-color IF and FISH staining to validate the select marker and MRV signal. (A) Expression levels of selected markers used to identify the selected cell subtypes. (B) Dual-color IF staining to validate the selected markers IgM, CD3, HSPb1, ACTA2 (green) and MRV (red) co-localization. The nucleus was stained with DAPI (blue), Scale bar, 50 μm. (C) Dual-color FISH and IF staining to validate the selected <t>markers</t> <t>CDH5,</t> ALOXE3, CSF1RB, <t>HBβ</t> (pink) and MRV (green) co-localization. Selected markers were identified by the corresponding probe and signal probe (CY5). MRV was identified by anti-MRV monoclonal antibody and secondary goat anti-mouse IgG antibody coupled with AlexaFluor 488 (green). The nucleus was stained by DAPI (blue), Scale bar, 50 μm. The arrows indicate the co-localization cells. (D) Transmission electron micrograph of five kinds of target cell, BC: blood cell, EC: endothelial cell, BM: basement membrane, CF: collagenous fiber, N: nucleus, Mit: mitochondria, ER: endoplasmic reticulum. The arrows indicated the virions. The scale is shown in the figure.
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    Dual-color IF and FISH staining to validate the select marker and MRV signal. (A) Expression levels of selected markers used to identify the selected cell subtypes. (B) Dual-color IF staining to validate the selected markers IgM, CD3, HSPb1, ACTA2 (green) and MRV (red) co-localization. The nucleus was stained with DAPI (blue), Scale bar, 50 μm. (C) Dual-color FISH and IF staining to validate the selected <t>markers</t> <t>CDH5,</t> ALOXE3, CSF1RB, <t>HBβ</t> (pink) and MRV (green) co-localization. Selected markers were identified by the corresponding probe and signal probe (CY5). MRV was identified by anti-MRV monoclonal antibody and secondary goat anti-mouse IgG antibody coupled with AlexaFluor 488 (green). The nucleus was stained by DAPI (blue), Scale bar, 50 μm. The arrows indicate the co-localization cells. (D) Transmission electron micrograph of five kinds of target cell, BC: blood cell, EC: endothelial cell, BM: basement membrane, CF: collagenous fiber, N: nucleus, Mit: mitochondria, ER: endoplasmic reticulum. The arrows indicated the virions. The scale is shown in the figure.
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    Dual-color IF and FISH staining to validate the select marker and MRV signal. (A) Expression levels of selected markers used to identify the selected cell subtypes. (B) Dual-color IF staining to validate the selected markers IgM, CD3, HSPb1, ACTA2 (green) and MRV (red) co-localization. The nucleus was stained with DAPI (blue), Scale bar, 50 μm. (C) Dual-color FISH and IF staining to validate the selected markers CDH5, ALOXE3, CSF1RB, HBβ (pink) and MRV (green) co-localization. Selected markers were identified by the corresponding probe and signal probe (CY5). MRV was identified by anti-MRV monoclonal antibody and secondary goat anti-mouse IgG antibody coupled with AlexaFluor 488 (green). The nucleus was stained by DAPI (blue), Scale bar, 50 μm. The arrows indicate the co-localization cells. (D) Transmission electron micrograph of five kinds of target cell, BC: blood cell, EC: endothelial cell, BM: basement membrane, CF: collagenous fiber, N: nucleus, Mit: mitochondria, ER: endoplasmic reticulum. The arrows indicated the virions. The scale is shown in the figure.

    Journal: bioRxiv

    Article Title: Dissection of the Global Responses of Mandarin Fish Pyloric Caecum to An Acute Ranavirus (MRV) Infection Reveals the Formation of Serositis and Then Ascites

    doi: 10.1101/2025.01.02.631049

    Figure Lengend Snippet: Dual-color IF and FISH staining to validate the select marker and MRV signal. (A) Expression levels of selected markers used to identify the selected cell subtypes. (B) Dual-color IF staining to validate the selected markers IgM, CD3, HSPb1, ACTA2 (green) and MRV (red) co-localization. The nucleus was stained with DAPI (blue), Scale bar, 50 μm. (C) Dual-color FISH and IF staining to validate the selected markers CDH5, ALOXE3, CSF1RB, HBβ (pink) and MRV (green) co-localization. Selected markers were identified by the corresponding probe and signal probe (CY5). MRV was identified by anti-MRV monoclonal antibody and secondary goat anti-mouse IgG antibody coupled with AlexaFluor 488 (green). The nucleus was stained by DAPI (blue), Scale bar, 50 μm. The arrows indicate the co-localization cells. (D) Transmission electron micrograph of five kinds of target cell, BC: blood cell, EC: endothelial cell, BM: basement membrane, CF: collagenous fiber, N: nucleus, Mit: mitochondria, ER: endoplasmic reticulum. The arrows indicated the virions. The scale is shown in the figure.

    Article Snippet: FISH sweAMI riboprobes and corresponding branch probe targeting the open reading frame (ORF) sequences of CDH5 , ALOXE3 , CSF1RB , HBβ and EPCAM genes were customized from Servicebio (Servicebiotech, China).

    Techniques: Staining, Marker, Expressing, Transmission Assay, Membrane